I-BlueKit ingumkhiqizi ohamba phambili kanye nomphakeli wemikhiqizo yokwelapha ngamaseli, ehlinzeka ngezinhlobonhlobo zezinsiza zokuthola i-ELISA zezinhlelo zokusebenza ezihlukahlukene. Phakathi kweminikelo yabo emisha i-293T HCP, ingxenye ebalulekile ekuthuthukisweni kwemikhiqizo yokwelapha ngamaselula. Kulokhu okuthunyelwe kwebhulogi, sizongena emhlabeni we-293T HCP futhi sihlole ukuthi iBlueKit iwuguqula kanjani umkhakha wokwelashwa kwamaseli.
Kwa-BlueKit, sisungule indlela eqondile yokuthuthukiswa kwemikhiqizo yokwelapha ngamaselula, kusukela ekutholakaleni kuya ekulethweni. Izinkundla zethu ezisezingeni eliphezulu zokukhiqiza i-nucleic acid, ukumiswa kwe-serum-free suspension culturing, kanye nokuthuthukiswa kwenqubo evalwe ngokuphelele kuqinisekisa amazinga aphezulu kakhulu emikhiqizo yethu. Ngobuchwepheshe bethu bokuhlola be-QC obuthuthukisiwe, siqinisekisa ukusebenza kahle nokuphepha kwawo wonke umkhiqizo onegama lomkhiqizo we-BlueKit.
Enye yeminikelo yethu evelele i-293T HCP, ingxenye ebalulekile ekukhiqizweni kwemikhiqizo yokwelapha ngamaseli. I-293T HCP idlala indima ebalulekile ekuthuthukisweni nasekukhiqizweni kwamaprotheni okwelapha, okwenza abacwaningi nososayensi bakhe izindlela zokwelapha ezintsha nezisebenzayo zezinhlobonhlobo zezifo. Nge-BlueKit's 293T HCP, abacwaningi bangathembela ekuthembekeni nasekunembeni kwemiphumela yabo, okuvula indlela yentuthuko edabukisayo emkhakheni wezokwelapha ngamaseli.
Ngokungeziwe ku-293T HCP, iBlueKit iphinde inikeze uhla lwezinsiza zokuhlonza i-ELISA zezinhlelo zokusebenza ezahlukahlukene ekwelashweni kwamaseli. Kusukela ekutholeni i-IL-15 eyinsalela yomuntu kuya ekulinganiseni ama-lentivirus titers, amakhithi ethu e-ELISA aklanyelwe ukwenza lula inqubo yokuthuthukisa futhi aqinisekise ikhwalithi nokuhlanzeka kwemikhiqizo yokwelapha ngamaseli. Ngamakhithi okuthola i-ELISA eBlueKit, abacwaningi bangaqhubekela phambili ngokuzethemba ocwaningweni lwabo, bazi ukuthi banokufinyelela kumathuluzi athuthuke kakhulu nathembekile emakethe.
Sengiphetha, iBlueKit ihamba phambili embonini yokwelapha ngamaseli, ihlinzeka ngemikhiqizo esezingeni eliphezulu kanye nobuchwepheshe ukuze isekele abacwaningi nososayensi emzamweni wabo wokuthuthukisa izindlela zokwelapha ezintsha. Ngamakhithi ethu okuhlonza we-293T HCP kanye ne-ELISA, sinikeza amathuluzi nezinsiza ezidingekayo ukuze kusheshiswe inqubo yokuthuthukisa nokuletha izindlela zokwelapha ezisindisa ukuphila kulabo abadinga usizo. Thembela ku-BlueKit kuzo zonke izidingo zakho zomkhiqizo wokwelashwa kwamaseli, futhi sindawonye, singaguqula ikusasa lokunakekelwa kwezempilo.
