I-BlueKit ngumvelisi ohamba phambili kunye nomthengisi weemveliso zonyango lweseli, enikezela uluhlu olubanzi lweekiti zokufumanisa i-ELISA kwizicelo ezahlukeneyo. Phakathi kweminikelo yabo entsha yi-293T HCP, icandelo eliphambili kuphuhliso lweemveliso zonyango lweselula. Kule post yebhlog, siza kungena kwihlabathi le-293T HCP kwaye sijonge ukuba iBlueKit iyiguqula njani intsimi yonyango lweeseli.
EBlueKit, siye saseka indlela ecacileyo yophuhliso lweemveliso zonyango lweselula, ukusuka ekufumaneni ukuya ekuhanjisweni. Amaqonga ethu okusika ekuveliseni i-nucleic acid, i-serum-free suspension culturing, kunye nophuhliso lwenkqubo evalwe ngokupheleleyo iqinisekisa eyona migangatho iphezulu yeemveliso zethu. Ngetekhnoloji yethu yovavanyo oluphezulu lwe-QC, siqinisekisa ukusebenza kunye nokhuseleko kuyo yonke imveliso enegama lohlobo lweBlueKit.
Omnye weminikelo yethu ebalaseleyo yi-293T HCP, icandelo elibalulekileyo kwimveliso yeemveliso zonyango lweeseli. I-293T HCP idlala indima ebalulekileyo ekuphuhliseni nasekuveliseni iiprotheyini zonyango, eyenza abaphandi kunye nososayensi benze unyango olutsha kunye olusebenzayo lwezifo ezahlukeneyo. NgeBlueKit's 293T HCP, abaphandi banokuthembela ekuthembekeni nasekuchanekeni kweziphumo zabo, bevula indlela yokuqhubela phambili kwintsimi yonyango lweeseli.
Ukongeza kwi-293T HCP, iBlueKit ikwabonelela ngoluhlu lweekiti zokufumanisa i-ELISA kwizicelo ezahlukeneyo kunyango lweeseli. Ukusuka ekufumaneni i-IL-15 yabantu eshiyekileyo ukuya kumlinganiso we-lentivirus titers, iikhithi zethu ze-ELISA ziyilelwe ukulungelelanisa inkqubo yophuhliso kunye nokuqinisekisa umgangatho kunye nokucoceka kweemveliso zonyango lweseli. Ngeekhithi zokubona ze-ELISA zeBlueKit, abaphandi banokuqhubela phambili ngokuzithemba kuphando lwabo, besazi ukuba banokufikelela kwezona zixhobo ziphambili nezithembekileyo kwimarike.
Ukuqukumbela, iBlueKit iphambili kumzi mveliso wonyango lweeseli, ibonelela ngeemveliso ezikumgangatho ophezulu kunye netekhnoloji yokuxhasa abaphandi kunye noosonzululwazi kumzamo wabo wokuphuhlisa unyango olutsha. Ngeekiti zethu ze-293T HCP kunye ne-ELISA, sinikezela ngezixhobo kunye nezixhobo ezifunekayo ukukhawulezisa inkqubo yophuhliso kunye nokuzisa unyango olusindisa ubomi kwabo basweleyo. Thembela kwiBlueKit kuzo zonke iimfuno zemveliso yonyango lweseli, kwaye kunye, sinokuliguqula ikamva lokhathalelo lwempilo.
