BlueKit ke moetsi ea ka sehloohong le morekisi oa lihlahisoa tsa kalafo ea lisele, e fanang ka mefuta e mengata ea lisebelisoa tsa ho lemoha ELISA bakeng sa lits'ebetso tse fapaneng. Har'a linyehelo tsa bona tse ncha ke 293T HCP, karolo ea bohlokoa ho nts'etsopele ea lihlahisoa tsa cellular therapy. Ka poso ena ea blog, re tla kenella lefats'eng la 293T HCP mme re hlahlobe hore na BlueKit e fetola joang lefapha la kalafo ea lisele.
Ho BlueKit, re thehile tsela e hlakileng ea nts'etsopele ea lihlahisoa tsa cellular, ho tloha ho sibolloa ho isa ho phano. Li-platform tsa rona tse tsoetseng pele tsa tlhahiso ea nucleic acid, temo ea ho emisoa ntle le serum, le nts'etsopele ea ts'ebetso e koetsoeng ka botlalo e netefatsa litekanyetso tse phahameng ka ho fetisisa tsa lihlahisoa tsa rona. Ka theknoloji ea rona e tsoetseng pele ea tlhahlobo ea QC, re netefatsa katleho le polokeho ea sehlahisoa se seng le se seng se nang le lebitso la BlueKit.
E 'ngoe ea linyehelo tsa rona tse hlahelletseng ke 293T HCP, karolo ea bohlokoa tlhahisong ea lihlahisoa tsa kalafo ea lisele. 293T HCP e bapala karolo ea bohlokoa ntlafatsong le tlhahisong ea liprotheine tsa kalafo, e nolofalletsang bafuputsi le bo-ramahlale ho etsa liphekolo tse ncha le tse sebetsang bakeng sa mafu a fapaneng. Ka BlueKit's 293T HCP, bafuputsi ba ka tšepa ho ts'epahala le ho nepahala ha liphetho tsa bona, ba bula tsela bakeng sa tsoelo-pele e kholo lefapheng la kalafo ea lisele.
Ntle le 293T HCP, BlueKit e boetse e fana ka lisebelisoa tse ngata tsa ho lemoha ELISA bakeng sa lits'ebetso tse fapaneng tsa kalafo ea lisele. Ho tloha ho fumana masala a IL-15 ho isa ho lekanya li-titer tsa lentivirus, lisebelisoa tsa rona tsa ELISA li etselitsoe ho tsamaisa tšebetso ea nts'etsopele le ho netefatsa boleng le bohloeki ba lihlahisoa tsa kalafo ea lisele. Ka lisebelisoa tsa ho bona tsa ELISA tsa BlueKit, bafuputsi ba ka tsoela pele ka kholiseho lipatlisisong tsa bona, ba tseba hore ba na le monyetla oa ho fumana lisebelisoa tse tsoetseng pele le tse tšepahalang 'marakeng.
Qetellong, BlueKit e ka pele indastering ea kalafo ea lisele, e fana ka lihlahisoa le mahlale a morao-rao ho ts'ehetsa bafuputsi le bo-ramahlale morerong oa bona oa ho nts'etsapele liphekolo tse ncha. Ka lisebelisoa tsa rona tsa ho lemoha tsa 293T HCP le ELISA, re fana ka lisebelisoa le lisebelisoa tse hlokahalang ho potlakisa ts'ebetso ea nts'etsopele le ho tlisa liphekolo tse pholosang bophelo ho ba hlokang. Tšepa BlueKit bakeng sa litlhoko tsohle tsa sehlahisoa sa hau sa kalafo ea lisele, 'me hammoho, re ka fetola bokamoso ba tlhokomelo ea bophelo bo botle.
