Sebakeng sa lipatlisiso tsa biomedical, bohlokoa ba li-reagents tsa boleng bo holimo bo ke ke ba fetisoa. Ho IPHASE, re ikemiselitse ho fana ka litharollo tse ncha bakeng sa bafuputsi lefatšeng ka bophara, 'me lisebelisoa tsa rona tsa ho itšehla thajana tsa PBMC ke karolo ea bohlokoa molemong oa sehlahisoa sa rona. Ka lihlahisoa tsa mantlha tse nang le tokelo ea molao tse fetang 600, re motlotlo ka bokhoni ba rona bo pharaletseng ba tlhahlobo ea lik'hemik'hale le baeloji, boenjineri ba DNA, le nts'etsopele ea protheine, ho netefatsa hore bareki ba rona ba fumana lisebelisoa tse ntle ka ho fetisisa bakeng sa boikitlaetso ba bona ba lipatlisiso.
Lisele tsa peripheral blood mononuclear (PBMCs) ke likarolo tsa bohlokoa ho immunology, lipatlisiso tsa mofets'e le libaka tse fapaneng tsa kalafo. Ho itšehla thajana ha li-PBMC ke mohato oa bohlokoa ho ithuteng karabelo ea 'mele ea ho itšireletsa mafung, ho utloisisa mekhoa ea mafu, le ho nts'etsapele liphekolo tse ncha. Ho IPHASE, re thehile lisebelisoa tsa rona tsa ho itšehla thajana tsa PBMC ho ntlafatsa chai le bohloeki ba lisele tsena tsa bohlokoa, ho thusa liphetho tse tšepahalang le tse ka hlahisoang litekong tsa hau. Lihlahisoa tsa rona li etselitsoe ho fihlela litlhoko tsa litsi tsa lipatlisiso, lik'hamphani tsa meriana, le mekhatlo ea lipatlisiso tsa konteraka (CROs), ho netefatsa hore bo-rasaense ba ka etsa mosebetsi oa bona ka kholiseho.
E 'ngoe ea matšoao a boitlamo ba rona ba boleng ke ts'ebetso ea rona e tiileng ea tlhahiso. Setsi se seng le se seng sa PBMC sa ho itšehla thajana se ntlafatsoa ka nepo e phahameng mme se latela litekanyetso tse thata tsa taolo ea boleng. Joaloka mofani oa thepa ea nang le botumo bo botle, re motlotlo ho fana ka li-CRO tse tummeng le lik'hamphani tsa meriana. Lethathamo la rona le pharalletseng la lihlahisoa le boetse le kenyelletsa lisebelisoa tsa boleng bo holimo tsa serum le plasma, joalo ka IPHASE Feline PPB Plasma le Rat (Sprague-Dawley) Serum kits, e fang bafuputsi likhetho tse eketsehileng bakeng sa lithuto tsa bona. Lisebelisoa tsena li tlatselletsa lihlahisoa tsa rona tsa ho itšehla thajana tsa PBMC, li fana ka lethathamo le felletseng la li-reagents bakeng sa lits'ebetso tse fapaneng.
Ho IPHASE, re utloisisa mathata ao bo-ramahlale ba tobanang le ona lefapheng la lipatlisiso le lulang le fetoha. Ke ka lebaka leo re lulang re tsetela ho R&D ho holisa lihlahisoa tsa rona le ho ntlafatsa litharollo tse teng. Bokhoni ba rona ba boqapi ba tlhahlobo ea immunoassays le cytogenetics bo matlafatsa bokhoni ba rona ba ho theha li-reagents tse tšehetsang matsapa a mangata a lipatlisiso. IPHASE Mouse CD11 Positive Selection Kit ke mohlala oa kamoo re hlokomelang litlhoko tse khethehileng tsa lipatlisiso ha re ntse re boloka boitlamo ba rona ba boleng le ts'ebetso.
Ha re sebeletsa bareki ba rona ba fetang 3,000 lefatšeng ka bophara, re amohela lepetjo la rona la "botšepehi, ho tiea, le ho sebetsa ka thata." Boinehelo bona ha bo bontše mekhoa ea rona ea khoebo feela empa hape bo ama sechaba sa bafuputsi haholo. Re lumela hore ka ho fana ka lihlahisoa tsa boleng bo holimo joalo ka lisebelisoa tsa rona tsa ho itšehla thajana tsa PBMC, re thusa ho matlafatsa bafuputsi ho ntšetsa pele kutloisiso ea mahlale le ho fihlela katleho mafapheng a bona a fapaneng.
Qetellong, ho khetha thepa e nepahetseng ea ho itšehla thajana ea PBMC ho bohlokoa bakeng sa laboratori efe kapa efe e batlang ho etsa lithuto tse tšepahalang tsa sesole sa 'mele. IPHASE e hlahella e le moetapele oa li-in-vitro biological reaagents, ka boitlamo ba boleng, boqapi le khotsofalo ea bareki. Hore na o ntse o etsa lipatlisiso ka karabelo ea 'mele ea ho itšireletsa mafung, o nts'etsapele liphekolo, kapa o etsa lipatlisiso tsa bongaka, lisebelisoa tsa rona tsa ho itšehla thajana tsa PBMC le mohala o pharalletseng oa lihlahisoa li tla tšehetsa boiteko ba hau le ho ntlafatsa bokhoni ba hau ba ho etsa lipatlisiso. Lekola linyehelo tsa rona 'me u fumane hore na IPHASE e ka u thusa joang ho fihlela lipheo tsa hau tsa lipatlisiso.
